


Received: OctoAccepted: FebruPublished: March 31, 2021Ĭopyright: © 2021 Jambor et al.

Swedlow, University of Dundee, UNITED KINGDOM PLoS Biol 19(3):Īcademic Editor: Jason R. (2021) Creating clear and informative image-based figures for scientific publications. Our recommendations address image magnification, scale information, insets, annotation, and color and may encourage discussion about quality standards for bioimage publishing.Ĭitation: Jambor H, Antonietti A, Alicea B, Audisio TL, Auer S, Bhardwaj V, et al. We present detailed descriptions and visual examples to help scientists avoid common pitfalls when publishing images. Papers that met all good practice criteria examined for all image-based figures were uncommon (physiology 16%, cell biology 12%, plant sciences 2%). Common problems included missing scale bars, misplaced or poorly marked insets, images or labels that were not accessible to colorblind readers, and insufficient explanations of colors, labels, annotations, or the species and tissue or object depicted in the image. We systematically examined these factors in non-blot images published in the top 15 journals in 3 fields plant sciences, cell biology, and physiology ( n = 580 papers). Many resources discuss fraudulent image manipulation and technical specifications for image acquisition however, data on the legibility and interpretability of images are scarce. Readers often examine figures first therefore, it is important that figures are accessible to a broad audience. Scientists routinely use images to display data.
